Table of Contents (Ver. 2.24.18)

I. Basic Experiments

  1. DNA digestion 
  2. DNA Ligation-1: User specified vector/insert ratio.
  3. DNA Ligation-2: Automatically calculate the vector/insert ratio.
  4. DNA Ligation-3: Automatically provide the range of vector/inset ratio from 1/1 to 1/5.
  5. DNA Assembly

II. Normalisation

  1. Normalisation by mass
  2. Normalisation by molecules
  3. Normalisation by cells

III. Conversion

  1. OD260 to mass
  2. OD260 to moles
  3. Mass to  moles
  4. Moles to mass
  5. RPM to RCF
  6. RCF to RPM

IV Physical quantities

  1. DNA physical quantities
  2. RNA physical quantities

V. Melting temperature

  1. Perfect matched DNA oligos
  2. Mismatched DNA oligos
  3. DNA oligos with inosines

VI. Plasmid, Cosmid, Phage

  1. Plasmid copy number
  2. Cosmid in vivo packaging
  3. Expected cosmid packaging efficiency
  4. Phage multiplicity of adsorption: Considering adsorption rate to evaluate multiplicity of infection (MOI).

VII. Bacterial growth

  1. Culture tracking
  2. OD alarm timer: Alert when the culture reaches the desired growth level.
  3. Fitness cost
  4. Conjugation rate

VIII. Lab strain references

  1. Selection antibiotics
  2. Genotypes
  3. Common markers
  4. Genes and products
  5. Essential genes

IX. Restriction enzymes

  1. Find recognition sequences
  2. Find Type IIP enzymes
  3. Find Type IIS enzymes
  4. Codon and enzymes
  5. Palindromic compatible ends